Search results for " DGGE"

BIODEGRADAZIONE DEGLI IDROCARBURI: DALLE COMUNITÀ MICROBICHE MARINE AI SISTEMI READY TO USE PER IL BIORISANAMENTO

Crude oil is one of the major hazardous pollutants. A promising technology for the treatment of contaminated sites is based on biodegradation of oil pollutants by microbial populations. Several microorganisms are known to use hydrocarbons as carbon sources. In the marine environment, obligate hydrocarbonoclastic bacteria (BIC) have been defined as being ecologically important for the aerobic removal of hydrocarbons. Recent advances in microbial ecology make it possible to combine molecular and culture-dependent approaches to identify bacterial community at environmental sites. In this thesis a microbiological survey of the Priolo Bay (eastern coast of Sicily, Ionian Sea), a chronically poll…

Estudio de la formación de biomasa granular en el tratamiento anaerobio de disolventes orgánicos en reactores de alta carga

2019

La contaminación atmosférica constituye uno de los principales problemas medioambientales en la actualidad, afectando a los ecosistemas y a la salud de los seres humanos a nivel mundial. Los compuestos orgánicos volátiles (COV), emitidos principalmente a partir del uso de disolventes en procesos industriales, contribuyen de manera importante a la contaminación atmosférica, razón por la cual, las industrias emisoras deben ajustarse a la normativa vigente, reduciendo sus emisiones y aplicando técnicas de control para el tratamiento y la eliminación de estos contaminantes atmosféricos. Las tecnologías biológicas se han mostrado eficaces a nivel técnico y económico para la depuración de las emi…

Rapid differentiation, in situ detection and monitoring of sourdough lactobacilli from the Abruzzo region (central Italy)

2006

Evolution of Ciona intestinalis Tumor necrosis factor alpha ( Ci TNFα): Polymorphism, tissues expression, and 3D modeling

2017

Although the Tumor necrosis factor gene superfamily seems to be very conserved in vertebrates, phylogeny, tissue expression, genomic and gene organization, protein domains and polymorphism analyses showed that a strong change has happened mostly in invertebrates in which protochordates were a constraint during the immune-molecules history and evolution. RT PCR was used to investigate differential gene expression in different tissues. The expression shown was greater in the pharynx. Single-nucleotide polymorphism has been investigated in Ciona intestinalis Tumor necrosis factor alpha (CiTNFα) mRNA isolated from the pharynx of 30 ascidians collected from Licata, Sicily (Italy), by denaturing …

Characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of Herculaneum in Italy

2011

Microbiological characterization of frescos in four different locations (Collegio degli Augustali, Casa del Colonnato Tuscanico, Casa dello Scheletro and Casa del Gran Portale) of excavation sites of Herculaneum was carried out. The use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. The microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming and mould. In fact, heterotrophic bacteria were prevalent in all wall paintings monitored (ranging from 18 +/- 2 CFU 100 cm(-2) to 68 +/- 4 CFU 100 cm(-2)), whereas fungi were also detected but at lowe…

Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

2006

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

Polymorphism of mytilin B mRNA is not traslated into mature peptide

2008

Diversity of mRNAs from mytilin B, one of the five mytilins identified in the Mediterranean mussel, Mytilus galloprovincialis, has been investigated from circulating hemocytes. One mussel expressed simultaneously two to ten different mytilin B mRNAs as observed in denaturing gradient gel electrophoresis (DGGE), defining 10 individual DGGE patterns (named A to J) within the mussels from Messina, Sicily (Italy). Three patterns accounted for 79% of the individuals whereas other patterns were found in only 2-7% of the 57 analyzed mussels. Base mutations were observed at specific locations, mainly within COOH-terminus and 3'UTR, leading to 36 nucleotide sequence variants and 21 different coding …

The occurrence of mycorrhizal fungi in Betula aetnensis Raf. roots: from ecological role to conservation strategies

2017

Betula aetnensis Raf. is an endemic tree species of particular conservation value. It only thrives in the north-eastern slopes of Mount Etna (Sicily), from 1200 to 2100 m a.s.l. This pioneer plant is able to begin primary succession on nutrient-poor and water-limited soils (C = 0.17%; N = 0.05 ‰; P2O5 = 4.1 ppm), where beneficial mycorrhizal fungi (MF) may play a crucial role. In order to investigate MF role in B. aetnensis, plant roots from natural sites and nursery grown specimens were analyzed for both ectomycorrhizal and endomycorrhizal structures. Typical structures of both symbiosis were detected by root staining and morphological observations. Ectomycorrhizae (EM) were more abundant …